XCR1 | Chemokine receptors | IUPHAR Guide to IMMUNOPHARMACOLOGY

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XCR1

Target id: 75

Nomenclature: XCR1

Family: Chemokine receptors

Annotation status:  image of a green circle Annotated and expert reviewed. Please contact us if you can help with updates.  » Email us

   GtoImmuPdb view: ON :     XCR1 has curated data in GtoImmuPdb

Gene and Protein Information
class A G protein-coupled receptor
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human 7 333 3p21.3-p21.1 XCR1 X-C motif chemokine receptor 1 5,10
Mouse 7 322 9 F4 Xcr1 chemokine (C motif) receptor 1 11
Rat 7 322 8q32 Xcr1 X-C motif chemokine receptor 1
Previous and Unofficial Names
GPR5 [5,10] | CCXCR1 | chemokine XC receptor 1 | C motif-1/lymphotactin receptor
Database Links
Specialist databases
GPCRDB xcr1_human (Hs), xcr1_mouse (Mm)
Other databases
ChEMBL Target
Ensembl Gene
Entrez Gene
Human Protein Atlas
KEGG Gene
OMIM
RefSeq Nucleotide
RefSeq Protein
UniProtKB
Wikipedia
Natural/Endogenous Ligands
XCL1 {Sp: Human}
XCL2 {Sp: Human}
XCL1 {Sp: Mouse} , XCL1 {Sp: Rat}

Download all structure-activity data for this target as a CSV file

Agonists
Key to terms and symbols Click column headers to sort
Ligand Sp. Action Affinity Units Reference
[125I]XCL1 (human) Hs Full agonist 8.0 pKd 10
pKd 8.0 [10]
XCL1 {Sp: Human} Hs Agonist ~7.3 pEC50 4
pEC50 ~7.3 (EC50 ~5x10-8 M) [4]
Description: Measuring Ca2+ flux
XCL2 {Sp: Human} Hs Agonist ~7.3 pEC50 4
pEC50 ~7.3 (EC50 ~5x10-8 M) [4]
Description: Measuring Ca2+ flux
Primary Transduction Mechanisms
Transducer Effector/Response
Gi/Go family Calcium channel
References:  10
Tissue Distribution
Placenta > spleen, thymus > peripheral blood leukocytes.
Species:  Human
Technique:  Northern blotting.
References:  10
Placenta > thymus > spleen > fetal thymus > fetal spleen > peripheral blood leukocytes > lymph node > bone marrow.
Species:  Human
Technique:  Dot blot.
References:  8
Neutrophils and T lymphocytes.
Species:  Mouse
Technique:  RT-PCR.
References:  2,6
B lymphocytes.
Species:  Mouse
Technique:  RT-PCR.
References:  6
Spleen, lung.
Species:  Mouse
Technique:  Northern blotting.
References:  11
CD8+ cells and NK1.1+ cells.
Species:  Mouse
Technique:  RT-PCR.
References:  11
Expression Datasets

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Log average relative transcript abundance in mouse tissues measured by qPCR from Regard, J.B., Sato, I.T., and Coughlin, S.R. (2008). Anatomical profiling of G protein-coupled receptor expression. Cell, 135(3): 561-71. [PMID:18984166] [Raw data: website]

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Functional Assays
Measurement of chemotaxis of T and B lymphocytes and neutrophils endogenously expressing the XCR1 receptor.
Species:  Mouse
Tissue:  T and B lymphocytes, neutrophils.
Response measured:  Chemotaxis.
References:  6
Measurement of Ca2+ levels in the murine pre-B cell line, L1.2, transfected with the human XCR1 receptor.
Species:  Human
Tissue:  L1.2 cells.
Response measured:  Ca2+ mobilisation.
References:  10
Measurement of chemotaxis of the murine pre-B cell line, L1.2, transfected with the human XCR1 receptor.
Species:  Human
Tissue:  L1.2 cells.
Response measured:  Chemotaxis.
References:  10
Measurement of Ca2+ levels in the murine pre-B cell line, B300-19, transfected with the mouse XCR1 receptor.
Species:  Mouse
Tissue:  B300-19 cells.
Response measured:  Ca2+ mobilisation.
References:  11
Measurement of chemotaxis of the murine pre-B cell line, B300-19, transfected with the mouse XCR1 receptor.
Species:  Mouse
Tissue:  B300-19 cells.
Response measured:  Chemotaxis.
References:  11
Physiological Functions
Chemotaxis.
Species:  Mouse
Tissue:  T and B lymphocytes, neutrophils.
References:  6
Recruitment of mononuclear cells to the rheumatoid arthritic joint.
Species:  Human
Tissue:  Synovial fluid of the rheumatoid joint.
References:  9
Phenotypes, Alleles and Disease Models Mouse data from MGI

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Allele Composition & genetic background Accession Phenotype Id Phenotype Reference
Xcr1tm1Dgen Xcr1tm1Dgen/Xcr1tm1Dgen
B6.129P2-Xcr1/J
MGI:1346338  MP:0002376 abnormal dendritic cell physiology PMID: 19913446 
Xcr1tm1Dgen Xcr1tm1Dgen/Xcr1tm1Dgen
involves: 129P2/OlaHsd * C57BL/6
MGI:1346338  MP:0002169 no abnormal phenotype detected

References

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1. Bachem A, G├╝ttler S, Hartung E, Ebstein F, Schaefer M, Tannert A, Salama A, Movassaghi K, Opitz C, Mages HW et al.. (2010) Superior antigen cross-presentation and XCR1 expression define human CD11c+CD141+ cells as homologues of mouse CD8+ dendritic cells. J. Exp. Med., 207 (6): 1273-81. [PMID:20479115]

2. Cairns CM, Gordon JR, Li F, Baca-Estrada ME, Moyana T, Xiang J. (2001) Lymphotactin expression by engineered myeloma cells drives tumor regression: mediation by CD4+ and CD8+ T cells and neutrophils expressing XCR1 receptor. J Immunol, 167: 57-65. [PMID:11418632]

3. Crozat K, Guiton R, Contreras V, Feuillet V, Dutertre CA, Ventre E, Vu Manh TP, Baranek T, Storset AK, Marvel J et al.. (2010) The XC chemokine receptor 1 is a conserved selective marker of mammalian cells homologous to mouse CD8alpha+ dendritic cells. J. Exp. Med., 207 (6): 1283-92. [PMID:20479118]

4. Fox JC, Nakayama T, Tyler RC, Sander TL, Yoshie O, Volkman BF. (2015) Structural and agonist properties of XCL2, the other member of the C-chemokine subfamily. Cytokine, 71 (2): 302-11. [PMID:25497737]

5. Heiber M, Docherty JM, Shah G, Nguyen T, Cheng R, Heng HH, Marchese A, Tsui LC, Shi X, George SR. (1995) Isolation of three novel human genes encoding G protein-coupled receptors. DNA Cell Biol, 14: 25-35. [PMID:7832990]

6. Huang H, Li F, Cairns CM, Gordon JR, Xiang J. (2001) Neutrophils and B cells express XCR1 receptor and chemotactically respond to lymphotactin. Biochem Biophys Res Commun, 281: 378-382. [PMID:11181058]

7. Lei Y, Takahama Y. (2012) XCL1 and XCR1 in the immune system. Microbes Infect., 14 (3): 262-7. [PMID:22100876]

8. Shan L, Qiao X, Oldham E, Catron D, Kaminski H, Lundell D, Zlotnik A, Gustafson E, Hedrick JA. (2000) Identification of viral macrophage inflammatory protein (vMIP)-II as a ligand for GPR5/XCR1. Biochem Biophys Res Commun, 268: 938-941. [PMID:10679309]

9. Stemmler S, Arinir U, Klein W, Rohde G, Hoffjan S, Wirkus N, Reinitz-Rademacher K, Bufe A, Schultze-Werninghaus G, Epplen JT. (2005) Association of interleukin-8 receptor alpha polymorphisms with chronic obstructive pulmonary disease and asthma. Genes Immun, 6: 225-230. [PMID:15772681]

10. Yoshida T, Imai T, Kakizaki M, Nishimura M, Takagi S, Yoshie O. (1998) Identification of single C motif-1/lymphotactin receptor XCR1. J. Biol. Chem., 273: 16551-16554. [PMID:9632725]

11. Yoshida T, Izawa D, Nakayama T, Nakahara K, Kakizaki M, Imai T, Suzuki R, Miyasaka M, Yoshie O. (1999) Molecular cloning of mXCR1, the murine SCM-1/lymphotactin receptor. FEBS Lett, 458: 37-40. [PMID:10518929]

Contributors

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How to cite this page

Israel F. Charo, Rebecca Hills, Richard Horuk, Kouji Matsushima, Philip M. Murphy, Joost J. Oppenheim.
Chemokine receptors: XCR1. Last modified on 19/02/2018. Accessed on 16/01/2019. IUPHAR/BPS Guide to PHARMACOLOGY, http://guidetoimmunopharmacology.org/GRAC/ObjectDisplayForward?objectId=75.