AFM13   Click here for help

GtoPdb Ligand ID: 10067

Synonyms: AFM 13 | AFM-13
Immunopharmacology Ligand
Compound class: Antibody
Comment: AFM13 is a tetravalent bispecific anti-CD30/CD16A tandem diabody that is constructed solely from Fv domains [1]. It is being developed by Affimed as a novel NK cell-engaging immuno-oncology therapeutic for the treatment of CD30-expressing malignancies. AFM13 simultaneously binds CD30 on Hodgkin's lymphoma cells and CD16A (FcγRIIIA) on NK cells, and acts to selectively recruit NK cells immune effector cells to destroy the cancer cells. AFM's mechanism of action differs from that of the anti-CD30 antibody-drug conjugate brentuximab vedotin, and it was found to be active in brentuximab vedotin-refractory patients in Phase 1 trial [2].
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Ligand Activity Visualisation Charts

These are box plot that provide a unique visualisation, summarising all the activity data for a ligand taken from ChEMBL and GtoPdb across multiple targets and species. Click on a plot to see the median, interquartile range, low and high data points. A value of zero indicates that no data are available. A separate chart is created for each target, and where possible the algorithm tries to merge ChEMBL and GtoPdb targets by matching them on name and UniProt accession, for each available species. However, please note that inconsistency in naming of targets may lead to data for the same target being reported across multiple charts.

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View interactive charts of activity data across species
Bioactivity Comments
Cytotoxicity of AFM13 is CD30-dependent and it does not bind the CD16B isoform [1].
Selectivity at catalytic receptors
Key to terms and symbols Click column headers to sort
Target Sp. Type Action Value Parameter Concentration range (M) Reference
CD30 Hs Antibody Binding 8.0 pKd - 1
⤷ pKd 8.0 (Kd 9.3x10-9 M) [1]
Description: Binding to CD30 determined in a competition assay on KARPAS-299 cells.
Selectivity at other protein targets
Key to terms and symbols Click column headers to sort
Target Sp. Type Action Value Parameter Concentration range (M) Reference
Fc fragment of IgG receptor IIIa Primary target of this compound Hs Antibody Binding 9.1 – 9.4 pKd - 1
⤷ pKd 9.1 – 9.4 (Kd 8.4x10-10 – 3.9x10-10 M) [1]
Description: Binding to CD16a 158V and 158F proteins by SPR.